Transient natural convection is numerically investigated in an enclosure using variable thermal conductivity, viscosity, and the thermal expansion coefficient of Al2O3-water nanofluid. The study has been conducted for a wide range of Rayleigh numbers (103≤ Ra ≤ 106), concentrations of nanoparticles (0% ≤ ϕ ≤ 7%), the enclosure aspect ratio (AR =1), and temperature differences between the cold and hot walls (∆T= 30). Transient parameters such as development time and time-average Nusselt number along the cold wall are also presented as a non-dimensional form. Increasing the Rayleigh number shortens the non-dimensional time of the initializing stage. By increasing the volume fraction of nanoparticles, the flow development time shows different behaviors for various Rayleigh numbers. The non-dimensional development time decreases by enhancing the concentration of nanoparticles.